Rutgers Researchers Discover Secrets of Nutritious Corn Breed That Withstands Rigors of Handling

Rutgers Researchers Discover Secrets of Nutritious Corn Breed That Withstands Rigors of Handling

Knowledge useful to improve hybrids that increase corn’s availability and nutrition in world food supplies

NEW BRUNSWICK, N.J. – Rutgers researchers have discovered the basis for
what makes corn kernels hard, a quality that allows corn to be easily
harvested, stored and transported. The findings could lead to better
hybrids and increase the supply for people in developing countries who
rely on it as a nutritional staple.

The discovery explains how a breed of corn known as “quality protein
maize,” or QPM, incorporates two qualities essential for an economical
and nutritious food crop: a source of key protein ingredients as well as
a hard-shelled kernel.

Until the arrival of QPM a decade ago, corn did not provide a balanced
protein mix when used as a sole food source. A hybrid developed in 1960
increased protein levels with essential amino acids but was commercially
unsuccessful, because its soft kernels subjected the harvest to

Joachim Messing

Joachim Messing

In a paper posted this week to the online early edition of the Proceedings of
the National Academy of Sciences
(PNAS), Rutgers geneticists reported
their findings about genetic coding responsible for making QPM kernels
sturdy. The sturdiness results from threshold levels of a specific gene
product encoded by two gene copies. Their investigation explains the
role of this gene product in generating a protein matrix around starch
particles that imparts seed strength.

“While QPM was developed in the late 1990s, scientists have not had a
thorough knowledge of how kernel strength could be achieved in a
rational way,” said Joachim Messing, professor of molecular genetics at
Rutgers. “Our work contributes knowledge that will help other scientists
develop better hybrids going forward, either through traditional
breeding techniques or genetic engineering.”

At the same time, the Rutgers findings will help scientists understand
more about the evolution of seeds and their components.

Corn is naturally low in lysine and tryptophan, amino acids that are
essential to make corn an adequate source of protein. Some societies
supplement corn with soybeans or other sources of protein in human food
and livestock feed. Yet there are societies, generally in South America
and Africa, where people rely on corn as their sole source of nutrition.

"QPM has made strides in overcoming malnutrition in these populations,
but to make it more available to people who need it, modern approaches
to breeding called ‘marker-assisted breeding’ will be superior in
adapting local corn varieties for these people,” said Messing, who is
also director of the Waksman Institute of Microbiology.

Research Plot

Yellow maize cobs harvested from a research plot in Mexico.

As part of the investigation, Rutgers postdoctoral researcher Yongrui Wu
used a technique to eliminate, or “knock out,” the expression of the
genes that geneticists suspected were involved in QPM kernel hardness.
After knocking out these two genes, responsible for producing proteins
known as gamma zeins, Wu observed softer kernels in the offspring.

Detailed investigation of original and knockout kernels using electron
microscopy revealed that soft kernels lacked a proteinaceous matrix
interconnecting starchy components while providing structural integrity.
Such structures were not present in the knockout offspring. The
researchers therefore pegged the gamma zeins regulated by these two
genes, labeled 16- and 27-kDa gamma zein, as key components of this
molecular structure and, as a result, QPM’s hardness.

The softer, commercially unsuccessful hybrid from 1960 had higher levels
of lysine and tryptophan because it had reduced levels of several
categories of zein proteins, which conferred kernel hardness but crowded
out other proteins that carried lysine and tryptophan. QPM has the
gamma zeins responsible for the hardness-preserving structure while
still lacking other zeins that crowded out nutritional proteins.

Collaborating with Wu and Messing was David Holding, assistant professor
of plant molecular genetics at the University of Nebraska-Lincoln. An
expert in genetic analysis of seeds, Holding provided a source of seeds
that were well-characterized for these studies.

The research was funded by the Selman A. Waksman Chair in Molecular
Genetics at Rutgers.


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